{ "generated_at": "2026-02-11T00:00:00Z", "source_lab_packets": "go_now_lab_packets.json", "source_redline_review": "go_now_feasibility_redlines.md", "criteria_version": "feasibility_go_no_go_v2_rfq_v1", "notes": [ "Prepared for quote solicitation; not a final statement of work until bidder Q&A closes.", "Crop genotype availability, shipping permits, and biosafety approvals must be confirmed before PO issuance.", "All quote recipients should return explicit attrition assumptions and event-level QC deliverables." ], "target_labs": [ { "id": "lab-danforth-plant-transformation", "name": "Danforth Center Plant Transformation Facility", "type": "fee_for_service_core", "fit_summary": "High fit for sorghum and switchgrass transformation, with academic/commercial service model.", "contact": { "primary": "Todd Finley", "email": "tfinley@danforthcenter.org", "intake_url": "" }, "source_evidence": [] }, { "id": "lab-mizzou-ptcf", "name": "University of Missouri Plant Transformation Core Facility", "type": "fee_for_service_core", "fit_summary": "Offers fee-based service for external/commercial users and supports sorghum/switchgrass transformation pathways.", "contact": { "primary": "Dr. Bing Yang", "email": "muptf@missouri.edu", "intake_url": "" }, "source_evidence": [] }, { "id": "lab-plantedits", "name": "PlantEdits LLC", "type": "commercial_cro", "fit_summary": "Commercial plant engineering provider with published transformation service scope and contact channel.", "contact": { "primary": "Commercial intake", "email": "contact@plantedits.com", "intake_url": "https://www.plantedits.com/pricing" }, "source_evidence": [ "https://www.plantedits.com/pricing" ] } ], "rfq_packages": [ { "rfq_id": "rfq-opc-go-001-v1", "source_experiment_id": "opc-go-001", "title": "RFQ: Editing Transformation-Competence Determinants in Recalcitrant Bioenergy Crop Genotypes", "objective": "Increase stable transformation efficiency in recalcitrant elite lines via targeted edits to competence-associated loci.", "scope_of_work": [ "Design/build up to 2 multiplex CRISPR constructs plus one non-targeting control (Wave 1 scope).", "Agrobacterium-mediated transformation in one transformable control genotype and up to two recalcitrant genotypes.", "Selection, regeneration, and event tracking to independent-event level.", "Molecular QC: target edit confirmation, transgene presence, copy-number estimate, backbone integration screen.", "Deliver analyzable event-level dataset and summary report." ], "client_provided_inputs": [ "Final prioritized locus list (4-6 loci) and preferred guide constraints", "Genotype list and seed/tissue source details", "Any existing validated vector backbones and selectable marker preferences", "Preferred reporting template and analysis conventions" ], "required_deliverables": [ "Independent event inventory with unique IDs and batch provenance", "Transformation efficiency table by genotype/construct/replicate", "Per-event QC table: target locus edit call, transgene status, copy estimate, backbone check", "Regeneration and survival metrics by arm", "Raw instrument/output files and processed tables in machine-readable format" ], "acceptance_criteria": [ "At least 8 independent PCR-positive events delivered per construct-genotype arm unless pre-agreed attrition exception is triggered", "Event-level QC table provided for all delivered events", "Matched-control analysis supports go/no-go call on >=2x efficiency lift criterion", "Any protocol deviations and failed batches documented with timestamps and root-cause notes" ], "quote_requirements": [ "Fixed fee by milestone and explicit variable-cost triggers", "Expected attrition model from explants to analyzable events", "Guaranteed minimum event count per arm and remediation path if under-delivered", "Turnaround for first analyzable dataset (calendar weeks)", "Permit/logistics responsibilities (who handles shipping/permits and at what cost)", "IP terms, data rights, and sample destruction/return policy" ], "timeline_request": { "rfq_issue_date": "2026-02-11", "questions_due": "2026-02-18", "quote_due": "2026-02-25", "target_kickoff": "2026-03-11" }, "recommended_targets": [ "lab-danforth-plant-transformation", "lab-mizzou-ptcf", "lab-plantedits" ] }, { "rfq_id": "rfq-opc-go-002-v1", "source_experiment_id": "opc-go-002", "title": "RFQ: Safe-Harbor Locus and Insulator Validation for Stable Transgene Expression", "objective": "Identify locus/insulator combinations that reduce between-line expression variance while preserving mean reporter expression.", "scope_of_work": [ "Wave 1 matrix: 2 candidate loci x (no-insulator + one insulator condition) plus random-integration comparator.", "Construct assembly and delivery using Agrobacterium pathway and agreed targeted insertion strategy.", "Event generation and pre-assay QC gating (junction PCR, copy estimate, locus confirmation).", "Expression assay execution (dual-luciferase and qPCR support) across defined T0/T1 tissue panel.", "Return line-level expression dataset and variance analysis-ready outputs." ], "client_provided_inputs": [ "Candidate safe-harbor coordinates and sequence context", "Insulator sequence set and cassette architecture preferences", "Reporter assay SOP preferences and normalization schema", "Target tissue panel for T0/T1 measurements" ], "required_deliverables": [ "Per-event insertion QC package (5' and 3' junction verification, copy estimate, locus call)", "Assay enrollment log showing which events passed QC gating", "Line-level ratiometric expression table with tissue, generation, and batch annotations", "Comparator analysis versus random integration arm", "Final recommendation matrix ranking locus/insulator combinations" ], "acceptance_criteria": [ "Minimum independent event counts per arm delivered per quoted commitment", "All analyzed lines have complete insertion QC records", "Returned dataset supports direct computation of between-line CV and stability metrics", "Bidder provides explicit caveats where power is insufficient for specific contrasts" ], "quote_requirements": [ "Detailed assumptions for targeted insertion success rates and rework policy", "Milestone-based cost breakdown including assay costs and failed-event handling", "Timeline to first T0 assay data and timeline to T1 follow-up", "Definition of deliverable formats (raw + processed), QA checks, and transfer mechanism", "Regulatory/biosafety handling responsibilities and exclusions", "IP/data rights, confidentiality, and publication clauses" ], "timeline_request": { "rfq_issue_date": "2026-02-11", "questions_due": "2026-02-18", "quote_due": "2026-02-25", "target_kickoff": "2026-03-18" }, "recommended_targets": [ "lab-danforth-plant-transformation", "lab-mizzou-ptcf", "lab-plantedits" ] } ], "outreach_templates": [ { "template_id": "email-rfq-opc-go-001", "subject": "RFQ Request: opc-go-001 crop transformation/editing study", "body": "Hello {lab_name},\n\nWe are requesting a quote for a staged crop transformation/editing study (RFQ ID: rfq-opc-go-001-v1).\n\nWe are attaching the scope, deliverables, and acceptance criteria. Please return:\n1) milestone-based pricing,\n2) expected event-yield/attrition assumptions,\n3) timeline to first analyzable dataset, and\n4) any required clarifications or exclusions.\n\nQuestions deadline: 2026-02-18\nQuote due: 2026-02-25\n\nThank you,\n{sender_name}\n{organization}\n{contact_info}" }, { "template_id": "email-rfq-opc-go-002", "subject": "RFQ Request: opc-go-002 safe-harbor/insulator validation", "body": "Hello {lab_name},\n\nWe are requesting a quote for a targeted insertion + expression stability study (RFQ ID: rfq-opc-go-002-v1).\n\nPlease review the attached scope and provide:\n1) milestone-based pricing,\n2) insertion success and attrition assumptions,\n3) timeline for T0 and T1 data delivery, and\n4) required client inputs or constraints.\n\nQuestions deadline: 2026-02-18\nQuote due: 2026-02-25\n\nThank you,\n{sender_name}\n{organization}\n{contact_info}" } ] }